At which phase do cells specifically ensure that the chromosomes are accurately separated into daughter cells?

The correct choice focuses on the phase known as Anaphase, during which the accurate separation of chromosomes into daughter cells is critically ensured.

During Anaphase, the sister chromatids, which have already been duplicated and aligned at the cell's equatorial plane during Metaphase, are pulled apart by the spindle fibers attached to the centromeres. This process is essential because it guarantees that each new daughter cell receives an identical set of chromosomes. The spindle apparatus plays a crucial role in this phase, as it pulls the chromatids toward opposite poles of the cell, ensuring that the genetic material is equally divided.

Other phases, such as Prophase, involve the condensation of chromatin into visible chromosomes and the formation of the spindle apparatus, but they do not focus directly on the separation of chromatids. Metaphase is primarily concerned with the alignment of chromosomes along the equator of the cell, and while it is critical for ensuring that all chromosomes are properly positioned before separation, it does not involve the actual splitting of chromatids. The G2 phase is a part of interphase dedicated to preparing for mitosis, including DNA repair and protein synthesis, rather than the separation of chromosomes.

Thus, Anaphase is the key phase where the